The influence of laser irradiation and glucose concentration on the content of carotenoids in the mycelium of fungus Laetiporus sulphureus (Bull.) Murrill
DOI:
https://doi.org/10.32999/ksu1990553X/2020-16-4-6Keywords:
mycelium, carotenoids, photoreception, photoactivation.Abstract
The article presents the results of the study of the content of carotenoids of L. sulphureus mycelium under the action of LED lasers: BRP–3010–5, with red spectrum radiation with a wavelength of 635 nm, BBP–3010–5 with blue spectrum radiation with a wavelength of 405 nm and BGP–3010–5 with green spectrum radiation with a wavelength of 532 nm (irradiation energy 51.1 mJ/cm2) when cultured on nutrient medium with different concentrations of glucose. The irradiated mycelium served as a control. It was found that is most effective for the synthesis of carotenoids the use of glucose-peptone medium with a glucose concentration of 10 g/dm³ in combination with irradiation of mycelium with green light at a wavelength of 532 nm (irradiation energy 51.1 mJ/cm2). Under the action of this irradiation regime for strain L.s.-18 the content of carotenoids in the mycelium increased by 66.1% according to the control. Laser irradiation of mycelium with blue light with a wavelength of 405 nm (irradiation energy 51.1 mJ/cm2) increased the content of carotenoids for strain L.s.-18 by 46.7%. Irradiation with red light with a wavelength of 635 nm (irradiation energy 51.1 mJ/cm2) contributed to an increase in the content of carotenoids for strain L.s.-16 of the fungus L. sulphureus by 28.9%. It was found that the use of glucose-peptone medium with a glucose concentration of 8 g/dm3 in combination with irradiation of the mycelium with green light with a wavelength of 532 nm (irradiation energy 51.1 mJ/cm2) was less effective. Under these conditions, the content of carotenoids in the mycelium increased for strain L.s.-17 by 62.3%. Laser irradiation of mycelium with blue light with a wavelength of 405 nm (irradiation energy 51.1 mJ/cm2) increased the content of carotenoids for strain L.s.-17 by 30.6%. Irradiation with red light with a wavelength of 635 nm (irradiation energy 51.1 mJ/cm2) contributed to an increase in the content of carotenoids for strain L.s.-18 of the fungus L. sulphureus by 16.8% respectively. For strain L.s.-16 the number of carotenoids in the mycelium no increase. The use glucose-peptone medium with glucose concentrations of 6 and 4 g/dm3 in combination with laser irradiation of mycelium with red (wavelength 635 nm), blue (wavelength 405 nm) and green (wavelength 532 nm) light with irradiation energy 51.1 mJ/cm2 was no increase in the content of carotenoids in the mycelium.
References
BECKER Z.E. (1988). Physiology and biochemistry of fungi. Moscow: Publishing house of Moscow University, 230 p. (in Russian)
BISKO N.A., BUKHALO A.S., WASSER S.P., DUDKA I.A., KULESH M.D., SOLOMKO E.F., SHEVCHENKO S.V. (1983). Higher edible basidiomycetes in surface and deep culture. K.: Science. Dumka, 312 p. (in Russian)
BRITTON G. (1986). Biochemistry of natural pigments. Per. from English Moscow: World, 442 p. (in Russian)
BUTSENKO L.M., PENCHUK YU.M., PYROG T.P. (2010). Technologies of microbial synthesis of drugs. Kyiv: NUHT, 323 p. (in Ukrainian)
CORROCHANO L.M., GARRE V. (2010). Photobiology in the Zygomycota: multiple photoreceptor genes for complex responses to light. Fungal Genet. Biol., 47: 893–899.
DE FABO E.C., FRIEDL M.A., SCHMOLL M., KUBICEK C.P., DRUZHININA I.S. (2008). Photostimulation of Hypocrea atroviridis growth occurs due to a crosstalk of carbon metabolism, blue light receptors and response to oxidative stress. Microbiology, 154: 1229–1241.
DUDKA I.A., WASSER S.P., ELLANSKAYA I.A. (1982). Methods of experimental mycology. Reference. Kyiv: Scientific opinion, 561 p. (in Russian)
ELDAHSHAN O.A., SINGAB A.N. (2013). Carotenoids. J. Pharmacogn. Phytochem. 2(1): 225–234.
FEDOTOV O.V. (2007). Wood-destroying fungi as bio-sources of ferments for medicinal and nutritional purposes. Plant and microbial enzymes: Isolation, characterization and biotechnology applications. Myza, Tbilisi, 125–131.
FROEHLICH A.C., NOH B., VIERSTRA R.D., LOROS J., DUNLAP J.C. (2005). Genetic and molecular analysis of phytochromes from the filamentous fungus Neurospora crassa. Eukaryot. Cell., 4: 2140–2152.
FULLER K.K., LOROS J.J., DUNLAP J.C. (2015). Fungal photobiology: visible light as a signal for stress, space and time. Curr Genet., 61: 275–288.
GESSLER N.N., SOKOLOV A.V., BYKHOVSKY V.YA., BELOZERSKAYA T.A. (2002). Superoxide dismutase and catalase activity in Blakeslea trispora and Neurospora crassa karatinoid-synthesizing fungi under conditions of oxidative stress. Applied Biochemistry and Microbiology, 8 (3): 237–242. (in Russian)
GESSLER N.N., OKOLOV A.B., BELOZERSKAYA T.A. (2003). Participation of ß-carotene in the antioxidant protection of the fungal cell. Applied Biochem. and Microbiol., 39 (4): 427–429. (in Russian)
GESSLER N.N., LEONOVICH O.A., RABINOVICH M.YA. (2006). A comparative study of the components of antioxidant defense during the growth of wild-type mycelium Neurospora crassa and mutants white color – 1 and white color – 2. Applied Biochemistry and Microbiology., 42 (3): 332–337. (in Russian)
GOODWIN T.W. (1980). The Biochemistry of carotenoids. Plants. Chapman & Hall, London, 1: 315 p.
KAMADA T., SANO H., NAKAZAWA T., NAKAHORI K. (2010). Regulation of fruiting body photomorphogenesis in Coprinopsis cinerea. Fungal Genet Biol., 47(11): 917–921.
KARNAUKHOV V.I. (1986). Biological functions of carotenoids. Moscow: Nauka, 223 p. (in Russian)
KRITSKIY M.S, TELEGINA T.A, VECHTOMOVA Y.L, KOLESNIKOVA M.P, LYUDNIKOVA T.A, GOLUB O.A. (2010). Photoexcited molecules of flavin and pterin coenzymes in evolution. Biochemistry., 75(10): 1348–1366.
MUSIENKO M.M., PARSHIKOVA T.V., SLAVNYI P.S. (2001). Spectrophotometric methods in the practice of physiology, biochemistry and plant ecology. Kyiv: Fitosotsiocenter, 200 p. (in Russian)
PIROG T.P., BUTSENKO L.M., PENCHUK Y.M. (2010). Technologies of microbial synthesis of drugs: Textbook. way. Kyiv: NUHT, 323 p. (in Ukrainian) POYEDYNOK N.L. (2013). The Use of artificial light in mushroom cultivation biotechnologies. Biotechnology Acta, 6(6): 58–70.
POYEDYNOK N.L. (2015). Biotechnological basis of intensification cultivation of edible and medicinal macromycetes with low light intensity. DSc thesis. Kyiv: Institute of Food Biotechnology and genomics. (in Russian)
PYROG T.P., IHNATOVA O.A. (2009). General biotechnology [Electronic resource]: textbook. Kyiv: NUHT, 336 p. (in Ukrainian)
PRYSEDSKYY Y.G. (2005). Software package for statistical processing of the results of the biological experiments. Donetsk, 84 р. (in Ukrainian)
RIBEIRO B., GUEDES DE PINHO P., ANDRADE P.B., OLIVEIRA C., CÉSAR A., FERREIRA S., BAPTISTA P., VALENTÃO DO P. (2011). Bioactive Carotenoids Contribute to the Color of Edible Mushrooms? The Open Chemical and Biomedical Methods Journal, 4: 14–18.
SAAKOV V.S. (2003). Alternative pathways of carotenoid biosynthesis in Procaryota and Eucaryota. Dokl. Academy of Sciences of Russia., 392(6): 825–831.
VELYGODSKA A.K., FEDOTOV O.V., PETREEVA A.S. (2014). Effect of nitrogen nutrition sources on carotenoids synthesis for some basidiomycetes strains. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 4(1): 22–34 (in Ukrainian).
VELYGODSKA A.K., FEDOTOV O.V. (2016). Obtaining and analysis of carotenoid preparations of some strains of xylotrophic basidiomycetes. Bulletin of Dnipropetrovsk University. Biology, ecology, 4 (2): 290–294. (in Ukrainian)
WETTSTEIN D. (1957). Chlorophyll-letale und der submikroskopishe Formweschsel der Plastiden. Exp Cell Res., 12(3): 427–506.
YU Z., FISCHER R. (2019). Light sensing and responses in fungi. Nature Reviews Microbiology, 17(1): 25–36.
ZHDANOVA N.N., VASILEVSKAYA A.I. (1982). Extreme ecology of mushrooms in nature and experiment. Kiev: Naukova Dumka, 168 p. (in Russian)
