The content of photosynthetic pigments in leaves of Berberis thunbergii forms in the Kherson region
DOI:
https://doi.org/10.32999/ksu1990-553X/2019-15-4-5Keywords:
Berberis, botanical garden, chlorophyll, carotenoids, Ukraine.Abstract
We carried out comparative studies of the photosynthetic pigments content in leaves of one- and two-year shoots in eight decorative forms of Berberis thunbergii DC. from the Agrobiostation – Botanical Garden collection of the Kherson State University. The chlorophyll content and carotenoids sum were defined in the leaves of these forms in July and early October, when there were no visual signs of leaf aging. The forms are differed by various colours of leaves: green, green with white-gray spots, purple, purple with yellow strip, purple with white-gray spots, yellow. In October, the chlorophyll content and carotenoids was higher than in the leaves of the same forms in July in the vast majority of forms. The maximum levels of the chlorophylls (2.51 mg/g) and carotenoids (0.89 mg/g) were found in green-coloured leaves with white-gray spots in the form “Kelleris” in October. In July, these rates were 2.11 mg/g and 0.64 mg/g respectively. The lowest content of the chlorophylls (0.22 mg/g) and carotenoids (0.11 mg/g) were in July in the leaves of two-year shoots of the yellow-leaf dwarf "Bananza gold". The content of photosynthetic pigments were generally higher in the leaves of one-year shoots vs. two- year ones. Ratio of chlorophyll a/chlorophyll b to chlorophyll sum/ carotenoids sum were varied during the growing season. It is varied from 1.0 to 3.1 and 1.0 to 3.3 in July and from 1.9 to 3.1 and from 1.3 to 4.0 in October. Usually, forms with green leaves had a higher content of the chlorophyll a and b and their sums vs. forms with purple and especially yellow leaves. This applies to the carotenoids sum in July also. In October, most carotenoids were found in leaves of the forms with purple leaf color, exception for the form “Kelleris”. The significant differences in the leaf colouring of decorative forms B. thunbergii are a real guarantee for the creation of colourful garden arrangements in parks and squares.
References
CHIKOV V.I. (2008). Evolyutsiya predstavleniy o svyazi fotosinteza s produktivnostyu rasteniy. Fiziologiya rasteniy, 55: 140–154. (in Russian) FEDENKO V.S. (2002). Vzaimosvyaz karotinoidnykh i fenolnykh pigmentov v formirovanii polikhroizma tsvetkov pokrytosemennykh rasteniy. Fiziologiya i biokhimiya kult. Rasteniy, 34 (3): 199–212. (in Russian)
IVANOV L.A., IVANOVA L.A., RONZHINA D.A., YUDINA P.K. (2013). Changes in the chlorophyll and carotenoid contents in the leaves of steppe plants along a latitudinal gradient in South Ural. Russian Journal of Plant Physiology, 60(6): 812–820. (in Russian) doi:10.7868/s0015330313050072
GILMORE A.M. (1997). Mechanistic aspects of xanthophylls cycledependent photoprotection in higher plant chloroplasts and leaves. Physiol. Plant, 99 (1): 197–209. doi: 10.1111/j.1399-3054. 1997.tb03449. x.
LICHTENTHALER H.K., BURKART S. (1999). Photosynthesis and high light stress. Bulg. J. Plant Physiol., 25 (3–4): 3–16.
MATSUBARA S., MOROSINOTTO T., OSMOND C.B., BASSI R. (2007). Shortand long-term operation of the lutein-epoxide cycle in light-haversting antenna complexes. Plant Physiol., 144 (2): 926–941. doi: 10.1104/pp. 107.099077
MERZLYAK M.N., GITELSON A.A. (1995). Why and What for the Leaves are Yellow in Autumn? On the Interpretation of Optical Spectra of Senescing Leaves (Acer platanoides L.). J. Plant. physiol., 145: 315–320.
MERZLYAK M.N. (1998). Pigmenty, optika lista i sostoyanie rasteniy. Sorosovskiy obrazovatelnyi zhurnal, 4: 19–24. (in Russian) doi: 10.1016/j.ecoenv.2009.01.010
SAVENKO A.V. CHUKURIDI S.S., BARCHUKOVA A.YA. (2015). Osobennosti adaptatsii sortov veygely (Weigela Thunb., Caprifoliaceae) v usloviyakh goroda Krasnodara. Politematicheskiy setevoy elektronnyi nauchnyi zhurnal Kubanskogo gosudarstvennogo agrarnogo universiteta, 1 (105): 726–738. (in Russian)
SIVASH A.A., ZOLOTAREVA E.K. (2013). Katabolizm khlorofilla v rasteniyakh. Visnik Kharkivskogo natsion. agrar. un-tu, 3 (30): 6–17. (in Russian) WELLBURN A.R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 144 (3): 307–313. doi: 10.1016/S0176-1617(11)81192-2
ZAPROMSTOV M.N. (1977). Metabolizm fenolnykh soedineniy v rasteniyakh. Biokhimiya, 42 (1): 3–20. (in Russian)
CROFT Y., CHEN J.M. (2017). Leaf pigment content. book: Reference module in earth systems and environmental sciences. Oxford: Elsevier Inc, 1–22.
HUGHES N.M, LEV-YADUN S. (2015). Red/purple leaf margin coloration: Potential ecological and physiological functions. Environmental and Experimetal Botany, 119: 27–39.
CHEN M. (2014). Chlorophyll modifications and their spectral extension in oxygenic photosynthesis. Annual Review of Biochemistry 83: 317–340.
GOULD K., MCKELVIE J., MARKHAM K. (2002). Do anthocyanins function as antioxidants in leaves? Imaging of H2O2 in red and green leaves after mechanical injury. Plant, Cell & Environment, 25: 1261–1269.
RICHARDSON A.D., DUIGAN S.P., BERLYN G.P. (2002). An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytologist 153: 185–194.
LUBELL J.D., BRAND M.H., LEHRER J.M., HOLSINGER K.E. (2008). Detecting the influence of ornamental Berberis thunbergii var. atropurpurea in invasive populations of Berberis thunbergii (Berberidaceae) using AFLP. American Journal of Botany, 95 (6): 1–7.
LUBELL J.D., BRAND M.H., LEHRER J.M. (2009). Amplified Fragment length polymorphism and parentage analysis of a feral Barberry (Berberis thunbergii DC.) population to determine the contribution of an ornamental landscape Genotype. Hortscience, 44 (2): 392–395.
ROMANOV M.V. (2008). Estimating the economic and biological properties of red-leaved and green-leaved forms of some plant species: specialty 06.01.05 – selection and seed production: dissertation abstract for the degree of candidate of agricultural sciences. Michurinsk. SOROKOPUDOV V.N., KHLEBNIKOV V.A. DEYNEKA V.I.( 2005). The anthocyanins of some plants of family Berberidaceae. Chemistry of plant raw materials. 4: 57–60.
